Figure 4

Oxidative stress as assayed by protein carbonyl content in fractions of striatal and cortical tissue. (a) There was a significant interaction between genotype and treatment in protein carbonyl content within the striatal mitochondria. Post hoc comparisons revealed that saline-HD mitochondria had higher protein carbonyl content than saline-WT and this was rescued in NAC-HD mice. (b–d) There were no differences in protein carbonyl content in the mitochondrial fraction of the cortex, or in the nuclear fractions from the striatum and cortex across either genotype or treatment groups. Error bars represent mean±s.e.m.; n=7–8; genotype and treatment effects: ^##P<0.01 and ^*P<0.05, respectively. HD, Huntington’s disease; NAC, N-acetylcysteine; WT, wild type.