Figure 1
From: Novel insights into the behavioral analysis of mice subjected to the forced-swim test
Customized setup for liquid exchange in the FST. (a) The diagram of the customized setup, showing the exchange inflow of the new liquid and the outflow of the original liquid in the container. (b) Two experiment protocols for the standard FST (6 min) and customized FST (8 min). (c and d) Exchanging water for 1 l of water (n=5) or 0.5% soap solution (n=5) using the customized setup. Fraction of total immobile time (Fim) (c) and angles (α) (d) of the animals both changed significantly after exchanging water for soap solution, going from epoch 1 (orange) to epoch 2 (green). The gray lines show the changes in individual mice from epoch 1 to epoch 2, and the colored filled circles show the averaged data in each epoch. FST, forced-swim test.
