Figure 4
From: Novel insights into the behavioral analysis of mice subjected to the forced-swim test
Probability of being mobile (Pmob) plots. (a) Probability of being mobile (Pmob) over the entire swim test shown in Figures 2e and f. The top two plots represent the binary states (M for mobile; I for immobile) of two individual mice with water (blue lines, top plot) or 1% soap solution (red lines, middle plot) applied to their caudal areas. The blue and red lines in the bottom plot represent the averages of the behavior, which is Pmob, of 11 mice in each group. The blue and red dashed lines show the averaged tlats calculated with tcs obtained for each mouse in the two groups. (b and c) Morlet wavelet transform of the Pmob plots shown in a, where color intensity indicates amplitude of a certain frequency component (y axis) as a function of time (x axis) of the Pmob plots. The rainbow color bar shows the corresponding color scale for the oscillation amplitudes in arbitrary units. A strong ‘oscillation’ in the swimming probability (green areas indicating a repeated stop-and-swim behavior with a certain frequency) with a comparable average frequency (0.060 and 0.067 Hz) occurred at similar times (174.2 s and 172.5 s) in the Pmob plots of mice regardless whether water (b) or 1% soap solution (c) was applied to their caudal areas. Even if the animals would have similar oscillating behavior, if they were out of phase with each other, the oscillating behavior would not appear in the averages, that is, the Pmob plots. tc, critical threshold; tlat, latency to immobility.
