Figure 5

Lifelong exercise changes the gene expression profile of Tg4-42^het mice. (a) Heatmap of differentially expressed genes (DEGs) between standard housing (SH) and enriched environment (EE) Tg4-42^het mice. Each column represents a pooled sample of two brain hemispheres and each row represents one of the top 60 genes that were differentially expressed. Yellow indicates upregulated and blue indicates downregulated gene expression. (b) Reverse transcription-PCR validation of deep sequencing results normalized to β-actin. (c) A protein-protein interaction network of genes found to be upregulated upon EE was created using STRING10 and showed enrichment of chaperones involved in endoplasmic reticulum protein processing. All data were given as means±s.e.m. *P<0.05, **P<0.01.