Figure 4 | Translational Psychiatry

Figure 4

From: Delineation of molecular pathway activities of the chronic antidepressant treatment response suggests important roles for glutamatergic and ubiquitin–proteasome systems

Figure 4

Differential effect of chronic paroxetine treatment on UPS. (a) Quantitative reverse transcription PCR data of NMDAR subunits, PSD-95 and nNOS showed no transcription differences between PLF and PSF groups, n=6 per group. (b) Immunoprecipitation (IP) study of ubiquitinated NMDAR subunits and PSD-95. IP with ubiquitin antibody was followed by western blot (WB) analysis of the proteins. No ubiquitinated protein level differences was observed between the PLF and PSF groups, n=3 per group. The proteins were blotted using total lysate extract. (c) PSMA2 and ubiquitination level differences between the PLF and PSF groups, n=5 per group. The proteins were blotted using cytoplasm-associated fraction. *P<0.05, **P<0.01, ***P<0.001 vs VIF, ##P<0.01, ###P<0.001 vs PLF (one-way analysis of variance (ANOVA) with Tukey’s test). Coomassie brilliant blue staining is shown as loading control. VIF mice were selected as a control group. NMDAR, N-methyl-d-aspartate receptor; nNOS, neuronal nitric oxide synthase; PLF, paroxetine-treated long-time floating; PSD-95, postsynaptic density protein-95; PSF, paroxetine-treated short-time floating; PSMA2, proteasome subunit α type-2; UPS, ubiquitin–proteasome system; VIF, vehicle-treated intermediate-time floating.

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