Volume 29 Issue 12, December 2022

Cover Credit: Although recombinant adeno-associated viruses (rAAVs) are conquering their way as one of the most promising gene delivery systems, the lack of imaging technologies to efficiently monitor their tropism at a whole-organ level with single-cell resolution remains challenging. In this issue of Gene Therapy, a new pipeline for the biodistribution analysis of natural and new variants of AAVs by tissue clearing and light-sheet fluorescence microscopy is explored. The immunofluorescence representative image shows the preferential tropism of rAAV9 encoding EGFP for Purkinje cells.