Using neutron reflectometry, AFM, and tensiometry, we analyzed AP2 binding to model membranes enriched in PtdIns(4,5)P2, unraveling Clathrin mediated endocytosis mechanisms. Cargo extends AP2 membrane residence, and AP2 interaction decreases phospholipid diffusion, potentially influencing clathrin binding.
- Armando Maestro
- Nathan R. Zaccai
- David J. Owen