Giardia lamblia is a human protozoan parasite with two diploid nuclei, which makes complete knock-out of a gene of interest challenging. Here the authors use a Cre/loxP-based approach to knock-out cyst-wall protein 1 (cwp1) and show that CWP1 is essential for cyst-wall biosynthesis.
- Jacqueline A. Ebneter
- Sally D. Heusser
- Carmen Faso
