Bio-based amphiphilic polymers having a helical hydrophobic unit have been extensively studied on molecular assemblies and their morphology. Molecular assemblies having complex morphologies could be prepared by using the specific characters of the helical unit, and the unique self-assembling methodology was named as ‘patchwork self-assembling’. Further, application of the obtained molecular assemblies in medicinal fields was examined. Lactosome was accumulated at the targeted tumor region by the EPR effect, and therefore, expected to be an excellent nano-ordered carrier for drug and/or imaging agent delivery.