Hydrophobicity plays an important role in protein function, but tuning hydrophobicity with canonical amino acids is chemically limited. Now through the genetic incorporation of bulky, highly hydrophobic non-canonical amino acids, their utility in enzyme engineering by enhancing the function of a copper-dependent laccase through hydrophobic tuning has been demonstrated.
- Sandro Fischer
- Anton Natter Perdiguero
- Alexandria Deliz Liang