Spatial transcriptomic approaches often lack single-cell resolution and read coverage. Here, we introduce spatial ISOform sequencing (Spl-ISO-Seq) which optimizes the capture of long, exonic molecules. This technology reveals developmentally-regulated cell-type and region-specific splicing patterns.
- Careen Foord
- Andrey D. Prjibelski
- Hagen U. Tilgner
