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Determination of G-protein–coupled receptor oligomerization by molecular brightness analyses in single cells

This protocol describes how to successfully design and execute molecular brightness analysis of G-protein–coupled receptor oligomerization. Labeling strategies, controls and instructions for spatial and temporal brightness imaging and data analysis are provided.

Ali Işbilir
Robert Serfling
Paolo Annibale
Protocols29 Jan 2021
Nature Protocols

Volume: 16, P: 1419-1451

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Determination of G-protein–coupled receptor oligomerization by molecular brightness analyses in single cells

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