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In vivo site-specific engineering to reprogram T cells

Stable and cell-specific transgene expression can be achieved through in vivo site-specific integration of large DNA payloads using a two-vector system of enveloped delivery vehicles and adeno-associated viruses.

William A. Nyberg
Pierre-Louis Bernard
Justin Eyquem
ResearchOpen Access18 Mar 2026
Nature

P: 1-10
SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci

Primary T cells with multiple genetic modifications are rapidly isolated by negative selection.

Christopher R. Chang
Vivasvan S. Vykunta
Justin Eyquem
ResearchOpen Access05 Feb 2025
Nature Biotechnology

Volume: 43, P: 2043-2053
Scalable intracellular delivery via microfluidic vortex shedding enhances the function of chimeric antigen receptor T-cells

Brandon J. Sytsma
Vincent Allain
Ryan S. Pawell
ResearchOpen Access17 Feb 2025
Scientific Reports

Volume: 15, P: 1-15