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Showing 1–13 of 13 results
Advanced filters: Author: Andrew R. Buller Clear advanced filters

  • Genetic rescue can be valuable for the conservation of small populations threatened by low genetic diversity, but it carries the perceived risk of outbreeding depression. Here, Weeks et al. report increased hybrid fitness in a rescued population of the mountain pygmy possum, which likely contributed to population growth following genetic rescue.

    • Andrew R. Weeks
    • Dean Heinze
    • Ian Mansergh
    ResearchOpen Access
    Nature Communications
    Volume: 8, P: 1-6

  • The intermolecular amination of C–H bonds is an enabling transformation for the synthesis of nitrogen-containing molecules; however, developing catalysts for this class of reactions is very challenging. Now, an iron-based enzyme for this reaction has been engineered, demonstrating that a protein can confer a difficult new function upon an otherwise unreactive base metal.

    • Christopher K. Prier
    • Ruijie K. Zhang
    • Frances H. Arnold
    Research
    Nature Chemistry
    Volume: 9, P: 629-634

  • Enantioselective C–C bond-forming reactions are underdeveloped in the biocatalysis toolbox. Now, engineering an efficient and promiscuous decarboxylative aldolase enzyme provides a solution to facilitate the convenient synthesis of non-standard γ-hydroxy amino acids from simple building blocks.

    • Jonathan M. Ellis
    • Meghan E. Campbell
    • Andrew R. Buller
    Research
    Nature Catalysis
    Volume: 5, P: 136-143

  • Efficient engineering of enzymes for expanded substrate scope is currently challenging. Here, the authors develop simple principles of how to design and interpret Substrate Multiplexed Screening assays to guide protein engineering to enable activity improvements with simultaneously with multiple substrates.

    • Allwin D. McDonald
    • Peyton M. Higgins
    • Andrew R. Buller
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-11

  • NocTE is a nonribosomal peptide synthetase thioesterase that completes the biosynthesis of pro-nocardicin G, the precursor for nocardicin β-lactam antibiotics. Here the authors provide mechanistic insights into NocTE by determining its crystal structures in the ligand-free form and covalently linked to a fluorophosphonate substrate mimic.

    • Ketan D. Patel
    • Felipe B. d’Andrea
    • Andrew M. Gulick
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-12

  • Aldolases have been a mainstay in synthesis, but their scope has been limited to activated electrophiles. Now carbon–carbon bond formation with ketone electrophiles is enabled by transaldolases, which form a strong nucleophile that is resistant to protonation. This chemistry enables convergent synthesis of non-canonical amino acids bearing tertiary alcohol side chains.

    • Samantha K. Bruffy
    • Anthony Meza
    • Andrew R. Buller
    Research
    Nature Chemistry
    Volume: 16, P: 2076-2083

  • Myelofibrosis is a risk factor for the development of Acute Myeloid Leukaemia. Here, the authors carry out an integrated genomic investigation of 933 myelofibrosis patients, and identified interactions between germline and somatic variation in patients who required haematopoietic cell transplantation.

    • Derek W. Brown
    • Weiyin Zhou
    • Mitchell J. Machiela
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-11

  • A protein–protein interface between a peptide-recognition domain (Fyn-SH3) and catechol O-methyltransferase (COMT) is computationally designed to generate a highly selective peptide-modifying system. Detailed mechanistic analysis sets a gold standard for studying the complex kinetic properties of designer fusion proteins.

    • Jonathan M. Ellis
    • Andrew R. Buller
    News & Views
    Nature Chemical Biology
    Volume: 19, P: 395-396

  • In an inter-laboratory study, the authors compare the accuracy and performance of three optical density calibration protocols (colloidal silica, serial dilution of silica microspheres, and colony-forming unit (CFU) assay). They demonstrate that serial dilution of silica microspheres is the best of these tested protocols, allowing precise and robust calibration that is easily assessed for quality control and can also evaluate the effective linear range of an instrument.

    • Jacob Beal
    • Natalie G. Farny
    • Jiajie Zhou
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-29