Hydrogen exchange–mass spectrometry (HX–MS) is used to qualitatively assess how perturbations such as mutations and ligand binding impact protein ensembles. However, in theory, HX–MS data contain the information necessary to derive residue-level energies of local unfolding (ΔGop). Now, a method called PIGEON-FEATHER has been developed, which can unambiguously determine residue-level ΔGop from conventional HX–MS datasets.
- Chenlin Lu
- Malcolm L. Wells
- Anum Glasgow
