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Showing 1–6 of 6 results
Advanced filters: Author: Avi I. Flamholz Clear advanced filters

  • A massively parallel assay developed to map the essential photosynthetic enzyme rubisco showed that non-trivial biochemical changes and improvements in CO2 affinity are possible, signposting further enzyme engineering efforts to increase crop yields.

    • Noam Prywes
    • Naiya R. Phillips
    • David F. Savage
    ResearchOpen Access
    Nature
    Volume: 638, P: 823-828

  • A genome-wide transposon screen in Halothiobacillus neapolitanus identifies genes involved in CO2 concentrating mechanisms, of which dabA and dabB encode a heterodimeric complex that works as an energy-coupled inorganic carbon pump. Dab homologues exist in multiple archaea and bacteria, including pathogens such as Bacillus anthracis and Vibrio cholerae.

    • John J. Desmarais
    • Avi I. Flamholz
    • David F. Savage
    Research
    Nature Microbiology
    Volume: 4, P: 2204-2215

  • In the construction of single fluorescent protein biosensors, selection of the insertion point of a fluorescent protein into a ligand-binding domain is a rate-limiting step. Here, the authors develop an unbiased, high-throughput approach, called domain insertion profiling with DNA sequencing (DIP-seq), to generate a novel trehalose biosensor.

    • Dana C. Nadler
    • Stacy-Anne Morgan
    • David F. Savage
    ResearchOpen Access
    Nature Communications
    Volume: 7, P: 1-11