Histidine phosphorylation is an elusive post-translational modification (PTM) whose role in mammalian cell biology is largely unknown due to the lack of robust tools and methods for its analysis. Here, the authors report the development of antibodies with unprecedented affinity and specificity towards 3-pHis and present the first crystal structures of a pHis peptide in complex with an antibody, showing how these antibodies can be used in standard molecular biology workflows to investigate pHis-dependent biology.
- Gregory D. Martyn
- Rajasree Kalagiri
- Sachdev S. Sidhu
