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Showing 1–6 of 6 results
Advanced filters: Author: Benjamien Moeyaert Clear advanced filters

  • The fluorescent chemogenetic reporters greenFAST and redFAST were engineered by protein engineering. They display orthogonal fluorogen recognition and spectral properties allowing efficient multicolor imaging of proteins in live cells and organisms.

    • Alison G. Tebo
    • Benjamien Moeyaert
    • Arnaud Gautier
    Research
    Nature Chemical Biology
    Volume: 17, P: 30-38

  • Combining the self-labeling HaloTag protein with synthetic environmentally sensitive fluorophores provides a platform for the construction of bright, far-red fluorescent calcium and voltage sensors with tunable photophysical and chemical properties.

    • Claire Deo
    • Ahmed S. Abdelfattah
    • Eric R. Schreiter
    Research
    Nature Chemical Biology
    Volume: 17, P: 718-723

  • Biosensors often report relative rather than absolute values. Here the authors report a method that utilises the photochromic properties of biosensors to provide an absolute measure of the analyte concentration or activity: photochromism-enabled absolute quantification (PEAQ) biosensing.

    • Franziska Bierbuesse
    • Anaïs C. Bourges
    • Peter Dedecker
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-13

  • Calcium imaging has been used to visualize the activity of individual synapses, but cannot be scaled up to monitor thousands of synapses in tissue. Here, the authors present genetic tools that can be photoconverted from green to red to create a map of active synapses.

    • Alberto Perez-Alvarez
    • Brenna C. Fearey
    • Thomas G. Oertner
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-16

  • Methods to directly label active neurons are still lacking. Here the authors develop CaMPARI2, a photoconvertible fluorescent protein sensor for neuronal activity with improved brightness and calcium binding kinetics, as well as an antibody to amplify the activated sensor signal in fixed samples.

    • Benjamien Moeyaert
    • Graham Holt
    • Eric R. Schreiter
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-12