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Showing 1–5 of 5 results
Advanced filters: Author: Benji Bateman Clear advanced filters

  • Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies between dyes on freely diffusing single molecules.

    • Benjamin Ambrose
    • James M. Baxter
    • Timothy D. Craggs
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-6

  • Particulate methane monooxygenase (pMMO) is the main enzyme used by methanotrophs. Here, the authors determined the native structure of pMMO by cryo-electron tomography, revealing lipid-stabilized features and a higher-order hexagonal array arrangement in intact cells.

    • Yanan Zhu
    • Christopher W. Koo
    • Peijun Zhang
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-10

  • In this study, Peijun Zhang and colleagues use cryoFIB/SEM volume imaging and soft x-ray cryo-tomography with cryo-electron tomography (cryoET) of cellular periphery, lamellae, and subtomogram averaging to place critical structural events in the SARS-CoV-2 infection cycle in the context of whole-cell images.

    • Luiza Mendonça
    • Andrew Howe
    • Peijun Zhang
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-10

  • Lin Wang et al. present a new super-resolution modality using a super-hemispherical immersion lens. They achieve a 12 nm spatial resolution in cells under cryogenic conditions, which offers the technical means to study bacterial and mammalian cell samples at molecule localisation length-scales.

    • Lin Wang
    • Benji Bateman
    • Marisa L. Martin-Fernandez
    ResearchOpen Access
    Communications Biology
    Volume: 2, P: 1-11

  • White et al show using quantitative imaging and optical tweezers that in tobacco leaf cells, the Miro2 GTPase promotes mitochondrial fusion and attachment to the ER. This is in contrast to metazoans where Miro mainly controls mitochondrial motility, with additional GTPases affecting fusion.

    • Rhiannon R. White
    • Congping Lin
    • Imogen Sparkes
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-8