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Showing 1–3 of 3 results
Advanced filters: Author: Birka Hein Clear advanced filters

  • A simple yet powerful super-resolution imaging approach based on switching off ordinary fluorophores and localizing those remaining or regaining fluorescence is illustrated using continuous widefield illumination and imaging of fixed and living cells labeled with rhodamine-derived dyes or fluorescent proteins. Biteen et al., also in this issue, describe related work using the ordinary fluorophore of EYFP for super-resolution imaging.

    • Jonas Fölling
    • Mariano Bossi
    • Stefan W Hell
    Research
    Nature Methods
    Volume: 5, P: 943-945

  • Here, subdiffraction-resolution STED fluorescence microscopy is used to detect the diffusion of single lipids or GPI-anchored proteins on the plasma membrane of a living cell. Tuning the probing spot area ∼70-fold below that of a confocal microscope reveals that unlike phosphoglycerolipids, sphingolipids and GPI-anchored proteins are trapped for ∼10 ms in cholesterol-mediated complexes within <20 nm space.

    • Christian Eggeling
    • Christian Ringemann
    • Stefan W. Hell
    Research
    Nature
    Volume: 457, P: 1159-1162