Advanced search

Filter By:

Journal Check one or more journals to show results from those journals only.

Choose more journals

Article type Check one or more article types to show results from those article types only.
Subject Check one or more subjects to show results from those subjects only.
Date Choose a date option to show results from those dates only.

Custom date range

Clear all filters
Sort by:
Showing 1–20 of 20 results
Advanced filters: Author: Björn Reinius Clear advanced filters

  • There are many open questions around the mechanism of sex chromosome dosage compensation in birds. In this study, Papanicolaou et al. show that female avian cells upregulate their single Z chromosome via increased transcriptional burst frequency and enhanced translation, revealing parallels with mammalian dosage compensation.

    • Natali Papanicolaou
    • Antonio Lentini
    • Björn Reinius
    ResearchOpen Access
    Nature Communications
    Volume: 16, P: 1-16

  • SARS-CoV-2 infection is widely diagnosed by RT-PCR, but RNA extraction is a bottleneck for fast and cheap diagnosis. Here, the authors develop protocols to perform RT-PCR directly on heat-inactivated subject samples or samples lysed with readily available detergents and benchmark performance against 597 clinically diagnosed patient samples.

    • Ioanna Smyrlaki
    • Martin Ekman
    • Björn Reinius
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-12

  • Rickard Sandberg and colleagues use allele-sensitive single-cell RNA–seq on primary mouse fibroblasts and human T cells to study clonal and dynamic monoallelic expression patterns. They find that the majority of random monoallelic expression of autosomal genes occurs transiently within individual cells rather than being stably inherited within clonally related cells.

    • Björn Reinius
    • Jeff E Mold
    • Rickard Sandberg
    Research
    Nature Genetics
    Volume: 48, P: 1430-1435

  • The concerted dynamics of X-chromosome upregulation and X-chromosome inactivation, which collectively balance X-chromosome expression, are not well understood. Using allelic single-cell genomics, the authors characterize the dynamics of X-chromosome upregulation and inactivation along mouse embryonic and stem cell development, calling to question keys aspects of the established model of mammalian dosage compensation.

    • Antonio Lentini
    • Huaitao Cheng
    • Björn Reinius
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-12

  • Effective RNase control is crucial in single-cell transcriptomics. Here, the authors introduce a synthetic, thermostable RNase inhibitor that enhances RNA stability and provides greater workflow flexibility in single-cell RNA-seq library preparation.

    • Joyce Carol Noble
    • Antonio Lentini
    • Björn Reinius
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-9

  • The Notch receptor is known to be activated by a pulling force, but whether it is strictly required remains to be clarified. Here, the authors demonstrate activation of Notch through soluble multivalent DNA origami constructs, showing effects in neuroepithelial-like stem cells.

    • Ioanna Smyrlaki
    • Ferenc Fördős
    • Björn Högberg
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-14

  • Analysis of X-chromosome upregulation using single-cell transcriptional kinetics data reveals increased burst frequency of X-linked genes that appear on the active X chromosome when X inactivation takes place.

    • Anton J. M. Larsson
    • Christos Coucoravas
    • Björn Reinius
    Research
    Nature Structural & Molecular Biology
    Volume: 26, P: 963-969

  • Allele-sensitive single-cell RNA sequencing analysis of long noncoding RNA (lncRNA) transcriptional kinetics shows that their lower expression compared to mRNA is due to lower burst frequencies and highlights cell-state-specific functions for several lncRNAs.

    • Per Johnsson
    • Christoph Ziegenhain
    • Rickard Sandberg
    ResearchOpen Access
    Nature Genetics
    Volume: 54, P: 306-317

  • DNA-origami-based insulin assembly into well-defined nanoclusters reveals that insulin valency and spatial organization modulate insulin receptor activation and downstream responses independent of ligand concentration.

    • Joel Spratt
    • José M. Dias
    • Ana I. Teixeira
    ResearchOpen Access
    Nature Nanotechnology
    Volume: 19, P: 237-245

  • The authors find a surprising diversity in hypothalamic neurons projecting to habenula, and using patch-sequencing (Patch-seq), identify an estrogen receptor-expressing neuron type that signals aversion and is linked to stress in female mice.

    • Daniela Calvigioni
    • Janos Fuzik
    • Konstantinos Meletis
    ResearchOpen Access
    Nature Neuroscience
    Volume: 26, P: 1245-1255

  • Allele-specific single-cell RNA sequencing provides insights into transcription kinetics, with data indicating that core promoter sequences affect burst size, whereas enhancers mainly affect burst frequency.

    • Anton J. M. Larsson
    • Per Johnsson
    • Rickard Sandberg
    Research
    Nature
    Volume: 565, P: 251-254

  • Long noncoding RNAs (lncRNAs) regulate gene expression by association with chromatin. Here, the authors show that lncRNA MEG3regulates the TGF-β pathway by bridging the interactions between polycomb repressive complex 2 and the distal regulatory elements of the TGF-β pathway genes via formation of RNA–DNA triplexes.

    • Tanmoy Mondal
    • Santhilal Subhash
    • Chandrasekhar Kanduri
    ResearchOpen Access
    Nature Communications
    Volume: 6, P: 1-17

  • In mammalian female germ cells, parent-specific epigenetic marks are erased and the X chromosome reactivated before entry into meiosis. Here, by combining parental haplotype reconstruction with single-cell transcriptomics of human female embryonic germ cells, the authors demonstrate that epigenetic reprogramming occurs in a heterogeneous fashion and during a broad time window up to week 14.

    • Ábel Vértesy
    • Wibowo Arindrarto
    • Susana M. Chuva de Sousa Lopes
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-10

  • In diploid organisms, expression from only one allele is frequently observed. This Review focuses on the widespread random monoallelic expression (RME) of autosomal genes, highlighting both the mitotically stable form observed in bulk analyses of cell populations and the recently discovered dynamic form identified through single-cell studies. The article also addresses the implications of different experimental criteria for calling monoallelic expression and potential biological roles in disease manifestations.

    • Björn Reinius
    • Rickard Sandberg
    Reviews
    Nature Reviews Genetics
    Volume: 16, P: 653-664