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Showing 1–27 of 27 results
Advanced filters: Author: Bridget Carragher Clear advanced filters

  • Current understanding of the structure of Dicer is restricted to simple forms of the enzyme from lower eukaryotes or isolated domains from higher eukaryotic Dicers. A new domain localization strategy was developed to determine the structure of human Dicer by EM, revealing the structural basis for small RNA production in eukaryotes.

    • Pick-Wei Lau
    • Keelan Z Guiley
    • Ian J MacRae
    Research
    Nature Structural & Molecular Biology
    Volume: 19, P: 436-440

  • Designed β-strand peptides stabilize integral membrane proteins for biochemical and structural studies, enabling electron microscopy analysis of the dynamic conformations of the ABC transporter MsbA.

    • Houchao Tao
    • Sung Chang Lee
    • Qinghai Zhang
    Research
    Nature Methods
    Volume: 10, P: 759-761

  • Time-resolved cryo-EM captured transient intermediates during E. coli RNAP promoter melting, revealing conformational changes affecting stepwise transcription bubble opening. Results inform how DNA sequence controls bacterial transcription initiation.

    • Ruth M. Saecker
    • Andreas U. Mueller
    • Seth A. Darst
    Research
    Nature Structural & Molecular Biology
    Volume: 31, P: 1778-1788

  • A standardized, realistic phantom dataset consisting of ground-truth annotations for six diverse molecular species is provided as a community resource for cryo-electron-tomography algorithm benchmarking.

    • Ariana Peck
    • Yue Yu
    • Mohammadreza Paraan
    ResearchOpen Access
    Nature Methods
    Volume: 22, P: 1819-1823

  • Two cryo-electron-microscopy images of the histone chaperone FACT interacting with components of nucleosomes shed light on how FACT manipulates nucleosomes to promote transcription, DNA repair and DNA replication.

    • Yang Liu
    • Keda Zhou
    • Karolin Luger
    Research
    Nature
    Volume: 577, P: 426-431

  • The cryo-EM structure of Mycobacterium smegmatis arabinosyltransferase B EmbB involved in mycobacterial cell wall biosynthesis provides insights into the substrate binding and reaction mechanism. Mapping of the ethambutol resistance associated mutations onto the structure suggests the location of the drug binding site.

    • Yong Zi Tan
    • José Rodrigues
    • Filippo Mancia
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-10

  • Here the authors describe the Waffle Method, aimed at increasing the throughput of and solves several challenges present in cryo-FIB/SEM sample preparation for cryo-ET analysis — the highest-resolution method for obtaining 3D views of native biological specimens in-situ.

    • Kotaro Kelley
    • Ashleigh M. Raczkowski
    • Alex J. Noble
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-13

  • Presequence protease (PreP) is essential to mitochondrial proteostasis. This study leverages advanced vitrification techniques to solve cryoEM structures of apo- and substrate-bound PreP and integrates these data with other analysis to reveal key stages and mechanistic insights of the PreP catalytic cycle.

    • Wenguang G. Liang
    • Juwina Wijaya
    • Wei-Jen Tang
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-14

  • Structural, functional and in silico analyses of the chloroquine-resistance transporter PfCRT of Plasmodium falciparum suggest that distinct mechanistic features mediate the resistance to chloroquine and piperaquine in drug-resistant parasites.

    • Jonathan Kim
    • Yong Zi Tan
    • Filippo Mancia
    Research
    Nature
    Volume: 576, P: 315-320

  • G protein-coupled receptors (GPCRs) are the largest family of transmembrane signaling proteins in humans and exhibit diverse activation mechanisms. Here, the authors combine electron microscopy, hydrogen deuterium exchange and Molecular dynamics simulations to gain insight into the activation mechanism of the glucagon receptor.

    • Linlin Yang
    • Dehua Yang
    • Hualiang Jiang
    ResearchOpen Access
    Nature Communications
    Volume: 6, P: 1-13

  • G protein-coupled receptors are a large family of signalling proteins that mediate cellular responses primarily via G proteins or arrestins, and they are targets of one-third of the current clinically used drugs; here, an active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin-1 is determined, revealing unique structural features that may constitute essential elements for arrestin-biased signalling.

    • Yanyong Kang
    • X. Edward Zhou
    • H. Eric Xu
    Research
    Nature
    Volume: 523, P: 561-567

  • This report describes the outcomes of the Data Management Challenges in 3D Electron Microscopy workshop. Key topics discussed include data models, validation and raw-data archiving. The meeting participants agreed that the EMDataBank should take the lead in addressing these issues, and concrete action points were agreed upon that will have a substantial impact on the accessibility of three-dimensional EM data in biology and medicine.

    • Ardan Patwardhan
    • José-Maria Carazo
    • Gerard J Kleywegt
    Comments & OpinionOpen Access
    Nature Structural & Molecular Biology
    Volume: 19, P: 1203-1207

  • A spraying-mixing approach for preparing cryo-EM grids using the Spotiton robot allows time-resolved observations of short-lived biomolecular states.

    • Venkata P. Dandey
    • William C. Budell
    • Bridget Carragher
    Research
    Nature Methods
    Volume: 17, P: 897-900

  • Clustered protocadherin ectodomains spontaneously assemble to form a zipper-like lattice of alternating cis and trans interactions at membrane contact sites, which probably represents their mode of function in neuronal self-recognition.

    • Julia Brasch
    • Kerry M. Goodman
    • Lawrence Shapiro
    Research
    Nature
    Volume: 569, P: 280-283

  • The preferred specimen orientation problem limits accuracy and resolution in structure determination by cryo-EM. Collecting data at defined sample tilts yielded near-atomic-resolution structures for the influenza hemagglutinin trimer and ribosomal biogenesis intermediates.

    • Yong Zi Tan
    • Philip R Baldwin
    • Dmitry Lyumkis
    Research
    Nature Methods
    Volume: 14, P: 793-796

  • Recent studies of the early secretory pathway have analysed cargo selection and transport-carrier formation by components of the endoplasmic-reticulum-associated coat protein complex-II (COPII). Results are indicative of a unifying model of cage and coat function in vesicle and tubule formation as well as fission in endomembrane traffic.

    • Cemal Gürkan
    • Scott M. Stagg
    • William E. Balch
    Reviews
    Nature Reviews Molecular Cell Biology
    Volume: 7, P: 727-738