In budding yeast, EcoI acetylates cohesin to establish sister chromatid cohesion in S phase. EcoI is subsequently modified by the ubiquitin E3 ligase SCF-Cdc4 and rapidly degraded. New work shows how sequential phosphorylation by three independent kinases generates a binding site for SCF-Cdc4 to promote EcoI degradation, thereby coupling EcoI activity, and thus sister chromatid cohesion, to the cell cycle.
- Nicholas A Lyons
- Bryan R Fonslow
- David O Morgan
