Different locations have been proposed for the catalytic centre of particulate methane monooxygenase for methane oxidation to methanol. Now, cryoelectron microscopy structures and electron nuclear double resonance spectroscopic measurements of the enzyme with a product analogue identify CuD as the active site and provide insights into substrate binding.
- Frank J. Tucci
- Richard J. Jodts
- Amy C. Rosenzweig