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Showing 1–13 of 13 results
Advanced filters: Author: Cedric A. J. Hutter Clear advanced filters

  • Here, the authors report the engineering, structural and biological characterization of synthetic nanobodies (sybodies) that display potent therapeutic activity against SARS-CoV-2 infection in animal models via targeting the virus receptor-binding domain.

    • Tingting Li
    • Hongmin Cai
    • Dianfan Li
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-13

  • Deep mutational scanning revealed the drug efflux activity profile of more than 1,430 single variants, enabling the identification of critical residues that regulate the activity of the bacterial drug efflux pump EfrCD in response to different drugs.

    • Gianmarco Meier
    • Sujani Thavarasah
    • Markus A. Seeger
    Research
    Nature Chemical Biology
    Volume: 19, P: 440-450

  • The mycobacterial ABC transporter IrtAB functions as a siderophore importer despite exhibiting an exporter fold in its structure, and contains a siderophore interaction domain capable of siderophore reduction and iron release inside the cell.

    • Fabian M. Arnold
    • Miriam S. Weber
    • Markus A. Seeger
    Research
    Nature
    Volume: 580, P: 413-417

  • Volume-regulated anion channels (VRACs) are heteromers of LRRC8 proteins, all containing the obligatory subunit LRRC8A. Here, the authors develop and characterize nanobodies that bind LRRC8A and allosterically modulate the function of homomeric LRRC8A and endogenous heteromeric channels, hinting at functional mechanisms present in VRACs.

    • Dawid Deneka
    • Sonja Rutz
    • Raimund Dutzler
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-14

  • ABC exporters hydrolyze ATP to pump substrates across membranes, but critical steps of the transport mechanism remain poorly understood. Here, the authors solve the crystal structure of outward-facing TM287/288 with the help of a state-specific sybody and gain insights into the role of the extracellular gate.

    • Cedric A. J. Hutter
    • M. Hadi Timachi
    • Markus A. Seeger
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-13

  • A method for protein binder selection and identification, NestLink, uses barcoding peptides detectable by mass spectrometry to select and biophysically characterize thousands of binders without requiring the handling of individual clones.

    • Pascal Egloff
    • Iwan Zimmermann
    • Markus A. Seeger
    Research
    Nature Methods
    Volume: 16, P: 421-428

  • Legionella pneumophila replicates in a Legionella-containing vacuole (LCV). Here the authors present the structure of the Legionella effector RidL N-terminal domain and reveal how RidL contributes to the subversion of retrograde trafficking by binding to the retromer coat complex subunit Vps29, which leads to a displacement of the regulator TBC1D5.

    • Kevin Bärlocher
    • Cedric A. J. Hutter
    • Hubert Hilbi
    ResearchOpen Access
    Nature Communications
    Volume: 8, P: 1-15

  • This protocol describes in vitro procedures for generation of synthetic single-domain antibodies called ‘sybodies’. Sybodies can be engineered to target specific protein conformations, labile membrane proteins or protein complexes.

    • Iwan Zimmermann
    • Pascal Egloff
    • Markus A. Seeger
    Protocols
    Nature Protocols
    Volume: 15, P: 1707-1741

  • Trinco et al. measure aspartate uptake rates in proteoliposomes containing purified prokaryotic Na+-coupled aspartate transporter GltTk. To overcome limitation of protein orientation, they use synthetic nanobody that blocks transporters from outside and reveal mechanistic features of Na+-aspartate symport that cannot be observed in detergent solution.

    • Gianluca Trinco
    • Valentina Arkhipova
    • Dirk J. Slotboom
    ResearchOpen Access
    Communications Biology
    Volume: 4, P: 1-11

  • In this work, the authors demonstrate that a coral thermostable GFP (TGP) is an improved tag compared to conventional GFPs. In addition to reporting melting point stability at temperatures near 90 °C, its fusion also helps increase expression levels of test membrane proteins. They further generated synthetic nanobodies against TGP to facilitate purification.

    • Hongmin Cai
    • Hebang Yao
    • Dianfan Li
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-16