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Showing 1–5 of 5 results
Advanced filters: Author: Chao-ting Wu Clear advanced filters

  • Synthetic oligonucleotides are the main cost factor for studies in DNA nanotechnology. Here, the authors present a selective oligonucleotide amplification method, based on three rounds of rolling-circle amplification, that produces nanomole amounts of single-stranded oligonucleotides per millilitre reaction.

    • Thorsten L. Schmidt
    • Brian J. Beliveau
    • William M. Shih
    ResearchOpen Access
    Nature Communications
    Volume: 6, P: 1-7

  • The spatial organization of the genome within the nucleus impacts many processes. Here the authors combine oligo-based DNA FISH with single-molecule super-resolution microscopy to image single-copy genomic regions and, taking advantage of SNPs, distinguish allelic regions of homologous chromosomes.

    • Brian J. Beliveau
    • Alistair N. Boettiger
    • Chao-ting Wu
    ResearchOpen Access
    Nature Communications
    Volume: 6, P: 1-13

  • Using super-resolution imaging to directly observe the three-dimensional organization of Drosophila chromatin at a scale spanning sizes from individual genes to entire gene regulatory domains, the authors find that transcriptionally active, inactive and Polycomb-repressed chromatin states each have a distinct spatial organisation.

    • Alistair N. Boettiger
    • Bogdan Bintu
    • Xiaowei Zhuang
    Research
    Nature
    Volume: 529, P: 418-422