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Showing 1–3 of 3 results
Advanced filters: Author: Charlotte Cautereels Clear advanced filters

  • Site-specific recombinases such as the Cre-LoxP system are routinely used for genome engineering in both prokaryotes and eukaryotes. Here the authors develop 63 symmetrical LoxP variants and test 1192 pairwise combinations to determine their cross-reactivity and specificity upon Cre activation.

    • Charlotte Cautereels
    • Jolien Smets
    • Kevin J. Verstrepen
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-15

  • In vivo mutagenesis allows improving cell factories through directed evolution. Here, the authors present CoMuTER, a tool for targetable random mutagenesis of complete biosynthetic pathways using a Cas3 enzyme fused to a cytidine deaminase.

    • Anna Zimmermann
    • Julian E. Prieto-Vivas
    • Kevin J. Verstrepen
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-16