Psoralen-conjugated triplex-forming oligonucleotides (Ps-TFOs) have been employed for the photodynamic regulation of gene expression by the photo-cross-linking of psoralen with the target DNA, however, thermodynamic stability remains a limitation, hampering the application of TFO. Here, the authors design a 1’(one)-psoralen-conjugated triplex-forming oligonucleotide to achieve stable parallel triplex formation and photo-cross-linking formation with pyrimidine-base-interrupting sequences under physiological conditions.
- Yu Mikame
- Haruki Toyama
- Asako Yamayoshi
