African swine fever virus type II DNA topoisomerase (AsfvTop2) is known to change DNA topology by employing a complex catalytic cycle and is involved in the early stages of viral gene replication, however, the structural and regulatory functions of AsfvTop2 remain underexplored. Here, the authors use cryo-EM to demonstrate six conformers of apo-AsfvTop2 as well as the structures of AsfvTop2-DNA-inhibitor complexes, revealing experimental evidence of their substrate binding mechanism that also shed light to other enzymes.
- Chiung-Wen Mary Chang
- Shun-Chang Wang
- Ming-Daw Tsai
