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Showing 1–5 of 5 results
Advanced filters: Author: Christian Kofoed Clear advanced filters

  • In this Tools of the Trade article, Kofoed (Muir lab) describes the development of the dual-component platform splicing-modulated actuation upon recognition of targets (SMART), which combines detection of cells with specific surface markers with a customizable output.

    • Christian Kofoed
    Research Highlights
    Nature Reviews Molecular Cell Biology
    Volume: 27, P: 91

  • A synthetic biology system called SMART has been developed that uses conditional protein splicing for the programmable ligation of functional proteins from previously defined molecular combinations on cell surfaces.

    • Christian Kofoed
    • Girum Erkalo
    • Tom W. Muir
    ResearchOpen Access
    Nature
    Volume: 645, P: 793-800

  • In an inter-laboratory study, the authors compare the accuracy and performance of three optical density calibration protocols (colloidal silica, serial dilution of silica microspheres, and colony-forming unit (CFU) assay). They demonstrate that serial dilution of silica microspheres is the best of these tested protocols, allowing precise and robust calibration that is easily assessed for quality control and can also evaluate the effective linear range of an instrument.

    • Jacob Beal
    • Natalie G. Farny
    • Jiajie Zhou
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-29