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Stimulated Raman scattering microscopy with a robust fibre laser source

A dual-wavelength fibre laser source has been developed for stimulated Raman scattering microscopy. It is precisely tunable over the entire high-wavenumber region of Raman spectra, where most stimulated Raman scattering imaging is performed. Imaging speeds of up to 1 frame s⁻¹ with shot-noise-limited sensitivity were achieved.

Christian W. Freudiger
Wenlong Yang
Khanh Q. Kieu
Research19 Jan 2014
Nature Photonics

Volume: 8, P: 153-159
Highly specific label-free molecular imaging with spectrally tailored excitation-stimulated Raman scattering (STE-SRS) microscopy

Spectral modulation of a broadband pump beam allows sensitive and specific molecular imaging based on stimulated Raman scattering. Measurements of cholesterol, protein, and stearic and oleic acid are reported.

Christian W. Freudiger
Wei Min
X. Sunney Xie
Research16 Jan 2011
Nature Photonics

Volume: 5, P: 103-109
RNAi screening for fat regulatory genes with SRS microscopy

Stimulated Raman scattering (SRS) microscopy is a quantitative, label-free imaging method to map fat distribution and accumulation with high spatial resolution and sensitivity at both cellular and organism levels.

Meng C Wang
Wei Min
X Sunney Xie
Research16 Jan 2011
Nature Methods

Volume: 8, P: 135-138
Localization of protoporphyrin IX during glioma-resection surgery via paired stimulated Raman histology and fluorescence microscopy

The clinical detection of fluorescent protoporphyrin IX during glioma-resection surgery can be improved via a microscope that pairs stimulated Raman histology and two-photon excitation fluorescence microscopy.

Mustafa Nasir-Moin
Lisa Irina Wadiura
Daniel A. Orringer
Research10 Jul 2024
Nature Biomedical Engineering

Volume: 8, P: 672-688
Foundation models for fast, label-free detection of glioma infiltration

FastGlioma is a visual foundation model for fast and accurate detection of glioma infiltration in fresh, unprocessed surgical tissue.

Akhil Kondepudi
Melike Pekmezci
Todd Hollon
ResearchOpen Access13 Nov 2024
Nature

Volume: 637, P: 439-445
Rapid intraoperative histology of unprocessed surgical specimens via fibre-laser-based stimulated Raman scattering microscopy

By taking advantage of stimulated Raman spectroscopy and fibre-laser technology, virtual histology images can be obtained in real time in the operating room, with diagnostic quality comparable with that achieved via conventional histopathology.

Daniel A. Orringer
Balaji Pandian
Sandra Camelo-Piragua
Research06 Feb 2017
Nature Biomedical Engineering

Volume: 1, P: 1-13
Artificial-intelligence-based molecular classification of diffuse gliomas using rapid, label-free optical imaging

DeepGlioma, a multimodal deep learning approach for intraoperative diagnostic screening of diffuse glioma, trained on stimulated Raman histology and large-scale public genomic data, can predict molecular alterations for diffuse glioma diagnosis with high accuracy.

Todd Hollon
Cheng Jiang
Daniel A. Orringer
Research23 Mar 2023
Nature Medicine

Volume: 29, P: 828-832
Near real-time intraoperative brain tumor diagnosis using stimulated Raman histology and deep neural networks

A prospective, multicenter, case–control clinical trial evaluates the potential of artificial intelligence for providing accurate bedside diagnosis of patients with brain tumors.

Todd C. Hollon
Balaji Pandian
Daniel A. Orringer
Research06 Jan 2020
Nature Medicine

Volume: 26, P: 52-58
Erratum: Multicolored stain-free histopathology with coherent Raman imaging

Christian W Freudiger
Rolf Pfannl
Geoffrey S Young
Amendments and Corrections05 Nov 2012
Laboratory Investigation

Volume: 92, P: 1661
Multicolored stain-free histopathology with coherent Raman imaging

Christian W Freudiger
Rolf Pfannl
Geoffrey S Young
Research20 Aug 2012
Laboratory Investigation

Volume: 92, P: 1492-1502

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