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Addendum to: Improved CRISPR genome editing using small highly active and specific engineered RNA-guided nucleases

Moritz J. Schmidt
Ashish Gupta
Wayne M. Coco
Amendments and CorrectionsOpen Access13 Jun 2023
Nature Communications

Volume: 14, P: 1
Genome-wide tracking of dCas9-methyltransferase footprints

Catalytically inactive Cas9 fused to a methyltransferase has emerged as a promising epigenome modifying tool. Here the authors generate a methylation depleted but maintenance competent mouse ES cell line and find ubiquitous off-target activity.

Christina Galonska
Jocelyn Charlton
Alexander Meissner
ResearchOpen Access09 Feb 2018
Nature Communications

Volume: 9, P: 1-9
Netrin-1 promotes naive pluripotency through Neo1 and Unc5b co-regulation of Wnt and MAPK signalling

Netrin-1, via precise Neo1/Unc5B stoichiometry, promotes naive pluripotency, embryonic stem cell self-renewal in combination with leukaemia inhibitory factor, and the formation of the mouse epiblast in vivo.

Aurélia Huyghe
Giacomo Furlan
Fabrice Lavial
Research30 Mar 2020
Nature Cell Biology

Volume: 22, P: 389-400
Dnmt1 has de novo activity targeted to transposable elements

The canonical DNA methylation maintenance enzyme Dnmt1 displays global de novo methylation activity with greater targeting towards IAP transposons, which may contribute to their stable repression during early development.

Chuck Haggerty
Helene Kretzmer
Alexander Meissner
ResearchOpen Access17 Jun 2021
Nature Structural & Molecular Biology

Volume: 28, P: 594-603
Improved CRISPR genome editing using small highly active and specific engineered RNA-guided nucleases

Small CRISPR Cas9 proteins have potential in gene therapies but generally have poor editing efficiency or specificity and often recognize sub-optimal PAM sequences. Here the authors characterise four small nucleases and use protein engineering to create effective in vivo editors.

Moritz J. Schmidt
Ashish Gupta
Wayne M. Coco
ResearchOpen Access09 Jul 2021
Nature Communications

Volume: 12, P: 1-12
Differential regulation of OCT4 targets facilitates reacquisition of pluripotency

Barriers underlying the inefficiency of reprogramming cells to pluripotency are poorly defined. Here the authors identify a transient interval soon after pluripotency exit that permits high-efficiency reprogramming and is facilitated by OCT4 bound elements displaying unique silencing behaviour during differentiation.

Sudhir Thakurela
Camille Sindhu
Alexander Meissner
ResearchOpen Access30 Sept 2019
Nature Communications

Volume: 10, P: 1-11
Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells

Alexander Meissner and colleagues use CRISPR/Cas9 genome editing to inactivate the DNA methyltransferases DNMT1, DNMT3A and DNMT3B in human embryonic stem cells (ESCs). They find an essential role for DNMT1 in human ESCs and generate genome-wide maps of the DNA methylation changes that occur following inactivation of these enzymes.

Jing Liao
Rahul Karnik
Alexander Meissner
Research30 Mar 2015
Nature Genetics

Volume: 47, P: 469-478
Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing

Expansions of short tandem repeats linked to neuropsychiatric disorders are measured by single-molecule sequencing.

Pay Giesselmann
Björn Brändl
Franz-Josef Müller
Research18 Nov 2019
Nature Biotechnology

Volume: 37, P: 1478-1481

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Addendum to: Improved CRISPR genome editing using small highly active and specific engineered RNA-guided nucleases

Genome-wide tracking of dCas9-methyltransferase footprints

Netrin-1 promotes naive pluripotency through Neo1 and Unc5b co-regulation of Wnt and MAPK signalling

Dnmt1 has de novo activity targeted to transposable elements

Improved CRISPR genome editing using small highly active and specific engineered RNA-guided nucleases

Differential regulation of OCT4 targets facilitates reacquisition of pluripotency

Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells

Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing

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