Advanced search

Filter By:

Journal Check one or more journals to show results from those journals only.

Choose more journals

Article type Check one or more article types to show results from those article types only.
Subject Check one or more subjects to show results from those subjects only.
Date Choose a date option to show results from those dates only.

Custom date range

Clear all filters
Sort by:
Showing 1–17 of 17 results
Advanced filters: Author: Christopher Kupitz Clear advanced filters

  • Here, the reaction of the suicide inhibitor sulbactam with the M. tuberculosis β-lactamase (BlaC) is investigated with time-resolved crystallography. Singular Value Decomposition is implemented to extract kinetic information despite changes in unit cell parameters during the time-course of the reaction.

    • Tek Narsingh Malla
    • Kara Zielinski
    • Marius Schmidt
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-15

  • The European X-ray free-electron laser (EuXFEL) in Hamburg is the first XFEL with a megahertz repetition rate. Here the authors present the 2.9 Å structure of the large membrane protein complex Photosystem I from T. elongatus that was determined at the EuXFEL.

    • Chris Gisriel
    • Jesse Coe
    • Nadia A. Zatsepin
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-11

  • Serial femtosecond X-ray crystallography permits the use of very small protein crystals; however, a continuous flow of sample is required. Weierstall et al. design and demonstrate an injector system that can supply microcrystals in the lipidic cubic phase, dramatically reducing the quantities of protein required.

    • Uwe Weierstall
    • Daniel James
    • Vadim Cherezov
    Research
    Nature Communications
    Volume: 5, P: 1-6

  • Serial femtosecond crystallography is an X-ray free-electron-laser-based method that uses X-ray bursts to determine protein structures. Here the authors present the structure of a photosynthetic reaction centre, an integral membrane protein, achieved with no sign of X-ray-induced radiation damage.

    • Linda C. Johansson
    • David Arnlund
    • Richard Neutze
    ResearchOpen Access
    Nature Communications
    Volume: 4, P: 1-7

  • Diacylglycerol kinase is a small bacterial membrane-bound trimer that catalyses diacylglycerol conversion to phosphatidic acid. Here, the authors solve the crystal structure of the kinase bound to a lipid substrate and an ATP analogue, and show that the active site arose through convergent evolution.

    • Dianfan Li
    • Phillip J. Stansfeld
    • Martin Caffrey
    ResearchOpen Access
    Nature Communications
    Volume: 6, P: 1-12

  • The new European X-Ray Free-Electron Laser (EuXFEL) is the first XFEL that generates X-ray pulses with a megahertz inter-pulse spacing. Here the authors demonstrate that high-quality and damage-free protein structures can be obtained with the currently available 1.1 MHz repetition rate pulses using lysozyme as a test case and furthermore present a β-lactamase structure.

    • Max O. Wiedorn
    • Dominik Oberthür
    • Anton Barty
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-11

  • Femtosecond X-ray pulses were used to obtain diffraction data on photosystem II, revealing conformational changes as the complex transitions from the dark S1 state to the double-pumped S3 state; the time-resolved serial femtosecond crystallography technique enables structural determination of protein conformations that are highly prone to traditional radiation damage.

    • Christopher Kupitz
    • Shibom Basu
    • Petra Fromme
    Research
    Nature
    Volume: 513, P: 261-265

  • G protein-coupled receptors are a large family of signalling proteins that mediate cellular responses primarily via G proteins or arrestins, and they are targets of one-third of the current clinically used drugs; here, an active form of human rhodopsin bound to a pre-activated form of the mouse visual arrestin-1 is determined, revealing unique structural features that may constitute essential elements for arrestin-biased signalling.

    • Yanyong Kang
    • X. Edward Zhou
    • H. Eric Xu
    Research
    Nature
    Volume: 523, P: 561-567

  • Although the photocycle of the photosensory core module of the Stigmatella aurantiaca bacteriophytochrome 2 (SaBphP2) has been extensively studied, its early dynamics have not been fully resolved. Here, the authors use time-resolved serial femtosecond crystallography to probe the associated picosecond events and report on the relative population of Z and E isomers after light activation.

    • Tek Narsingh Malla
    • Luis Aldama
    • Marius Schmidt
    ResearchOpen Access
    Communications Chemistry
    Volume: 8, P: 1-10

  • Crystal lattice disorder, which gives rise to a continuous diffraction pattern, is exploited to determine the structure of the integral membrane protein complex photosystem II to a higher resolution than could be achieved using Bragg diffraction alone.

    • Kartik Ayyer
    • Oleksandr M. Yefanov
    • Henry N. Chapman
    Research
    Nature
    Volume: 530, P: 202-206

  • Ayan et al. report two structures of the protein streptavidin - one at ambient temperature determined using serial femtosecond crystallography and a second one determined at cryogenic temperature. These results provide insights into the structural dynamics of apo streptavidin and reveal a cooperative allostery between monomers for binding to biotin, and the findings are supported by GNM analysis.

    • Esra Ayan
    • Busra Yuksel
    • Hasan DeMirci
    ResearchOpen Access
    Communications Biology
    Volume: 5, P: 1-13