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Showing 1–10 of 10 results
Advanced filters: Author: Christopher M Roome Clear advanced filters

  • Dendritic integration is important for information processing in the brain. Here, in awake mice, authors combine simultaneous dendritic recording of voltage and calcium signals, with somatic recording from Purkinje neurons, enabling characterization of dendritic spiking, action potential backpropagation, and ‘hotspots’ in spiny dendrites.

    • Christopher J. Roome
    • Bernd Kuhn
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-14

  • The local X-ray-induced dynamics that occur in protein crystals during serial femtosecond crystallography (SFX) measurements at XFELs are not well understood. Here the authors performed a time-resolved X-ray pump X-ray probe SFX experiment, and they observe distinct structural changes in the disulfide bridges and peptide backbone of proteins; complementing theoretical approaches allow them to further characterize the details of the X-ray induced ionization and local structural dynamics.

    • Karol Nass
    • Alexander Gorel
    • Ilme Schlichting
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-9

  • X-ray fee-electron lasers (XFELs) enable time-resolved crystallography experiments and the structure determination of proteins with little or no radiation damage. However currently it is unknown whether the designated 4.5 MHz maximum pulse rate for the European XFEL could lead to sample damage caused by shock waves from preceding pulses. Here, the authors address this question by performing a X-ray pump X-ray probe experiment on haemoglobin microcrystals at the Stanford XFEL facility that mimics the 4.5 MHz data collection mode and observe structural changes and a drop in diffraction data quality of the crystals.

    • Marie Luise Grünbein
    • Alexander Gorel
    • Ilme Schlichting
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-11

  • The European X-ray free-electron laser (EuXFEL) in Hamburg is the first megahertz (MHz) repetition rate XFEL. Here the authors use lysozyme crystals and microcrystals from jack bean proteins and demonstrate that damage-free high quality data can be collected at a MHz repetition rate.

    • Marie Luise Grünbein
    • Johan Bielecki
    • Ilme Schlichting
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-9

  • rsEGFP2 is a reversibly photoswitchable fluorescent protein used in super-resolution light microscopy. Here the authors present the structure of an rsEGFP2 ground-state intermediate after excited state-decay that was obtained by nanosecond time-resolved serial femtosecond crystallography at an X-ray free electron laser, and time-resolved absorption spectroscopy measurements complement their structural analysis.

    • Joyce Woodhouse
    • Gabriela Nass Kovacs
    • Martin Weik
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-11

  • Bacteriorhodopsin (bR) is a light-driven proton pump. Here the authors combine time-resolved crystallography at a free-electron laser, ultrafast spectroscopy and quantum chemistry to study the structural changes following multiphoton photoexcitation of bR and find that they occur within 300 fs not only in the light-absorbing chromophore but also in the surrounding protein.

    • Gabriela Nass Kovacs
    • Jacques-Philippe Colletier
    • Ilme Schlichting
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-17

  • X-ray free-electron lasers produce bright femtosecond X-ray pulses. Here, the authors use a two-colour X-ray free-electron laser beam for simultaneous two-wavelength data collection and show that protein structures can be determined with multiple wavelength anomalous dispersion phasing, which is important for difficult-to-phase projects.

    • Alexander Gorel
    • Koji Motomura
    • Ilme Schlichting
    ResearchOpen Access
    Nature Communications
    Volume: 8, P: 1-8

  • Providing detailed structural descriptions of the ultrafast photochemical events that occur in light-sensitive proteins is key to their understanding. Now, excited-state structures in the reversibly switchable fluorescent protein rsEGFP2 have been solved by time-resolved crystallography using an X-ray laser. These structures enabled the design of a mutant with improved photoswitching quantum yields.

    • Nicolas Coquelle
    • Michel Sliwa
    • Martin Weik
    Research
    Nature Chemistry
    Volume: 10, P: 31-37