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High-throughput peptide-centric local stability assay extends protein–ligand identification to membrane proteins, tissues and bacteria

Li et al. further develop a high-throughput peptide-centric local stability assay that speeds up sample preparation 100-fold and extends protein–ligand identification to membrane proteins, tissues and bacteria.

Kejia Li
Clement M. Potel
Mikhail M. Savitski
ResearchOpen Access05 Nov 2025
Nature Structural & Molecular Biology

Volume: 33, P: 184-192
Uncovering protein glycosylation dynamics and heterogeneity using deep quantitative glycoprofiling (DQGlyco)

Here the authors present deep quantitative glycoprofiling (DQGlyco), a method that enables high-throughput analysis of protein glycosylation dynamics. Using DQGlyco, they link gut microbiome composition to brain glycoproteins and provide insights into glycoform functionality and tissue specificity.

Clément M. Potel
Mira Lea Burtscher
Mikhail M. Savitski
ResearchOpen Access10 Feb 2025
Nature Structural & Molecular Biology

Volume: 32, P: 1111-1126
Impact of phosphorylation on thermal stability of proteins

Clément M. Potel
Nils Kurzawa
Mikhail M. Savitski
Research17 Jun 2021
Nature Methods

Volume: 18, P: 757-759
Protein-Peptide Turnover Profiling reveals the order of PTM addition and removal during protein maturation

Metabolic labeling is often used to measure protein turnover. Here the authors show that for interconvertible protein species like phosphoforms metabolic labeling does not provide information on turnover differences, but that the relative order of modification can determine the observed dynamics.

Henrik M. Hammarén
Eva-Maria Geissen
Mikhail M. Savitski
ResearchOpen Access02 Dec 2022
Nature Communications

Volume: 13, P: 1-15
Systematic discovery of biomolecular condensate-specific protein phosphorylation

A combination of solubility proteome profiling with phosphoproteomics enables systematic analysis of the phosphorylation status of proteins in soluble and condensate-bound pools.

Sindhuja Sridharan
Alberto Hernandez-Armendariz
Mikhail M. Savitski
ResearchOpen Access21 Jul 2022
Nature Chemical Biology

Volume: 18, P: 1104-1114
High-throughput functional characterization of protein phosphorylation sites in yeast

Phenotypic profiling of a phospho-deficient mutant library informs functional annotations of phosphosites.

Cristina Viéitez
Bede P. Busby
Pedro Beltrao
Research18 Oct 2021
Nature Biotechnology

Volume: 40, P: 382-390
Widespread bacterial protein histidine phosphorylation revealed by mass spectrometry-based proteomics

A twist on a common method used for enriching phosphorylated peptides for mass spectrometry-based proteomics analysis now reveals previously undetected and widespread histidine phosphorylation in Escherichia coli.

Clement M Potel
Miao-Hsia Lin
Simone Lemeer
Research29 Jan 2018
Nature Methods

Volume: 15, P: 187-190

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High-throughput peptide-centric local stability assay extends protein–ligand identification to membrane proteins, tissues and bacteria

Uncovering protein glycosylation dynamics and heterogeneity using deep quantitative glycoprofiling (DQGlyco)

Impact of phosphorylation on thermal stability of proteins

Protein-Peptide Turnover Profiling reveals the order of PTM addition and removal during protein maturation

Systematic discovery of biomolecular condensate-specific protein phosphorylation

High-throughput functional characterization of protein phosphorylation sites in yeast

Widespread bacterial protein histidine phosphorylation revealed by mass spectrometry-based proteomics

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