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Advanced filters: Author: Conor A. Treacy Clear advanced filters

  • Förster resonance energy transfer (FRET) is essential for studying molecular interactions, yet challenges remain in mapping complex interactions in cellular environments. Here, the authors employ a three-fluorophore FRET-cascade system with time-correlated single photon counting fluorescence lifetime imaging microscopy to elucidate Rap1-interacting adaptor molecule (RIAM)-vinculin interactions in focal adhesions, revealing mechanosensitive roles of vinculin and its interaction with RIAM in a force-independent manner.

    • Conor A. Treacy
    • Tommy L. Pallett
    • Simon M. Ameer‑Beg
    ResearchOpen Access
    Communications Chemistry
    Volume: 9, P: 1-12