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Showing 1–6 of 6 results
Advanced filters: Author: Daniel L. Coutu Clear advanced filters

  • This resource paper describes the steps involved in carrying out quantitative multicolour imaging in tissue. It is applied to cleared mouse bone and plots the spatial distribution of specific cell populations within the marrow.

    • Daniel L Coutu
    • Konstantinos D Kokkaliaris
    • Timm Schroeder
    Research
    Nature Methods
    Volume: 15, P: 39-46

  • Vascularization is critical for cranial bone growth, maintenance, and healing, but it remains unknown how blood vessels are spatially distributed in the calvarium, and how they interact with skeletal progenitors during these processes. Here, the authors apply a quantitative light-sheet imaging platform to visualize and analyze the relationship between blood vessels and skeletal progenitors throughout the murine calvarium.

    • Alexandra N. Rindone
    • Xiaonan Liu
    • Warren L. Grayson
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-13

  • Live imaging and single-cell analyses are used to show that decision-making by differentiating haematopoietic stem cells between the megakaryocytic–erythroid and granulocytic–monocytic lineages is not initiated by stochastic switching between the lineage-specific transcription factors PU.1 and GATA1, which challenges the previous model of early myeloid lineage choice.

    • Philipp S. Hoppe
    • Michael Schwarzfischer
    • Timm Schroeder
    Research
    Nature
    Volume: 535, P: 299-302

  • Schroeder and colleagues review the development and application of single-cell technologies — from gene and protein expression to clonal labelling, lineage tracing and time-lapse imaging — in stem cell research.

    • Philipp S. Hoppe
    • Daniel L. Coutu
    • Timm Schroeder
    Reviews
    Nature Cell Biology
    Volume: 16, P: 919-927