The authors report a toolkit for multiplexed quantification of CRISPR/Cas9-mediated DSBs intermediates and repair products by single-molecule sequencing: they characterise the dynamics of DSB induction, processing, and repair in tomato protoplasts, showing that precise repair is a prominent feature of the repair process.
- Daniela Ben-Tov
- Fabrizio Mafessoni
- Avraham A. Levy