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Showing 1–3 of 3 results

Advanced filters: Author: Daphne S. Bindels Clear advanced filters

Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

Marieke Mastop
Daphne S. Bindels
Joachim Goedhart
ResearchOpen Access20 Sept 2017
Scientific Reports

Volume: 7, P: 1-18
mScarlet: a bright monomeric red fluorescent protein for cellular imaging

An extremely bright, truly monomeric RFP, mScarlet, is described that outperforms existing RFPs in diverse labeling applications, especially in FRET with ratiometric imaging.

Daphne S Bindels
Lindsay Haarbosch
Theodorus W J Gadella Jr
Research21 Nov 2016
Nature Methods

Volume: 14, P: 53-56
Multiparameter screening method for developing optimized red-fluorescent proteins

This protocol describes a screening pipeline to select red fluorescent proteins (FPs) with optimized parameters from a mutant FP library. FPs are screened for fluorescence lifetime, cellular brightness, maturation efficiency, and photostability.

Daphne S. Bindels
Marten Postma
Theodorus W. J. Gadella Jr
Protocols15 Jan 2020
Nature Protocols

Volume: 15, P: 450-478