The RGS family is involved in regulating G protein signaling. Using computational analysis coupled with examination of RGS activity, a group of variable residues that together modulate G protein recognition are now identified. Mutational analysis confirmed the importance of these modulatory residues and generated gain-of-function RGS proteins. The analysis described is shown to be applicable to uncovering important residues in other protein families.
- Mickey Kosloff
- Amanda M Travis
- Vadim Y Arshavsky
