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Nature Biotechnology (2)
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Advanced filters: Author: Emma W. Vaimberg Clear advanced filters

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

Genome-wide sgRNA libraries based on rules for on-target activity improve results of Cas9-based screens and facilitate a further refinement of on- and off-target prediction algorithms.

John G Doench
Nicolo Fusi
David E Root
Research18 Jan 2016
Nature Biotechnology

Volume: 34, P: 184-191
Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

Genome-wide libraries for CRISPR knockout, interference, and activation have allowed the systemic interrogation of gene function. Here, the authors evaluate the Brunello CRISPRko library and introduce Dolcetto and Calabrese for CRISPRi and CRISPRa, respectively.

Kendall R. Sanson
Ruth E. Hanna
John G. Doench
ResearchOpen Access21 Dec 2018
Nature Communications

Volume: 9, P: 1-15
Orthologous CRISPR–Cas9 enzymes for combinatorial genetic screens

Combinatorial CRISPR screens are improved by using Cas9 enzymes from two different organisms.

Fadi J Najm
Christine Strand
John G Doench
Research18 Dec 2017
Nature Biotechnology

Volume: 36, P: 179-189