SpCas9 off-targets are a safety concern. Here the authors report a cleavage rule that governs the on-target and off-target cleavage of increased(/high)-fidelity SpCas9 variants: the variants have differences in fidelity small enough to comprise an optimal variant for each target, irrespective of its cleavability ranking.
- Péter István Kulcsár
- András Tálas
- Ervin Welker
