Diterpene glycosyltransferase UGT76G1 is a key enzyme in the biosynthesis of rebaudioside A, a naturally sweet-tasting compound, however, its poor thermostability hinders its industrial application. Here, the authors use a computational design strategy to generate a mutant of UGT76G1 that exhibits improved thermostability and enzymatic activity.
- Seong-Ryeong Go
- Su-Jin Lee
- Eui-Jeon Woo
