Heparan sulfate can promote formation of amyloid fibrils by different proteins. The early steps and kinetics of this process are now studied in detail, using a stopped-flow setup and the protein mAcP. The data and analysis reveal two steps, binding of mAcP to heparan sulfate and conversion to a misfolded state, followed by aggregation of the heparan sulfate–bound proteins.
- Neda Motamedi-Shad
- Tommaso Garfagnini
- Fabrizio Chiti
