The E3 ubiquitin ligases RNF8 and RNF168 are required for recruitment of tumor surpressor 53BP1 to sites of DNA double-strand breaks. The reasons for this have been unclear, as 53BP1 recognizes histone mark H4K20me2. Now the AAA-ATPase VCP and cofactors are shown to be recruited in a ubiquitination-dependent manner to double-strand break sites, where they remove polycomb protein L3MBTL1 from chromatin.
- Klara Acs
- Martijn S Luijsterburg
- Nico P Dantuma
