This paper reports a chemical genetics strategy for regulating a developmental protein in a fetus in a reversible, time-controlled fashion. Remarkably, this control can be achieved in the mother's uterus. An existing chemically regulated allele of glycogen synthase kinase-3β (GSK-3β) is used, where the protein is fused to a tag, and its activity depends on the presence of rapamycin, which destabilizes the mutant.
- Karen J. Liu
- Joseph R. Arron
- Michael T. Longaker