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Showing 1–10 of 10 results
Advanced filters: Author: Ghader Bashiri Clear advanced filters

  • Cofactor F420 plays crucial roles in bacterial and archaeal metabolism, but its biosynthetic pathway is not fully understood. Here, the authors present the structure of one of the enzymes and provide experimental evidence for a substantial revision of the pathway, including the identification of a new intermediate.

    • Ghader Bashiri
    • James Antoney
    • Colin J. Jackson
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-12

  • Pepstatins are potent inhibitors of aspartic proteases, featuring two statine residues crucial for target binding, however, their biosynthesis is elusive. Here, the authors discover and characterize an unconventional gene cluster responsible for pepstatin biosynthesis, and characterize the role of PepI, an F420H2-dependent oxidoreductase catalysing the tandem reduction of β-keto pepstatin intermediates.

    • Jingjun Mo
    • Asfandyar Sikandar
    • Chengzhang Fu
    ResearchOpen Access
    Nature Communications
    Volume: 16, P: 1-14

  • Poly-γ-glutamate tails are a distinctive feature of folate and F420 cofactors, but it was unclear how these tails elongate while maintaining substrate specificity. Here, the authors discover that folylpolyglutamate synthase and γ-glutamyl ligase enzymes add successive L-glutamates to the termini of the growing γ-glutamyl chain in a processive mechanism.

    • Ghader Bashiri
    • Esther M. M. Bulloch
    • Christopher J. Squire
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-11

  • Isocitrate lyase (ICL) isoforms 1 and 2 are enzymes in the glyoxylate and methylcitrate cycles that enable Mycobacterium tuberculosis (Mtb) to use lipids as a carbon source. Here the authors present the ligand-free Mtb ICL2 and acetyl-CoA bound ICL2 crystal structures, which reveal a structural reorganisation upon acetyl-CoA binding that leads to an activation of its isocitrate lyase and methylcitrate lyase activities.

    • Ram Prasad Bhusal
    • Wanting Jiao
    • Ivanhoe K. H. Leung
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-7

  • β-Nicotinamide adenine dinucleotide is used as a building block in secondary metabolite biosynthetic pathways for a novel class of natural products.

    • Lena Barra
    • Takayoshi Awakawa
    • Ikuro Abe
    Research
    Nature
    Volume: 600, P: 754-758

  • Macrocyclization is an important process in bioactive natural product synthesis. Here, the authors report on the study of a macrocyclic ring constructing enzyme in the biosynthesis of alchivemycin A and using gene deletion, biochemical assays and isotope labelling show the enzyme catalyses tandem dehydration and Michael-type addition.

    • Hong Jie Zhu
    • Bo Zhang
    • Hui Ming Ge
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-11

  • Epoxide ring opening reactions are important in both biological processes and synthetic applications. Here, the authors show that flavin cofactors can catalyze reductive and oxidative epoxide ring opening reactions and propose the underlying mechanisms.

    • Bidhan Chandra De
    • Wenjun Zhang
    • Changsheng Zhang
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-13