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Showing 1–4 of 4 results
Advanced filters: Author: Gregory Bokinsky Clear advanced filters

  • Free-living microorganisms regulate the fluidity of their membranes by adapting lipid composition to environmental temperatures. Here, the authors quantify lipid biosynthetic enzymes and metabolic intermediates to provide a quantitative description of how the bacterium Escherichia coli maintains constant membrane fluidity after a temperature shock.

    • Loles Hoogerland
    • Stefan Pieter Hendrik van den Berg
    • Gregory Bokinsky
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-13

  • HIV-1 reverse transcriptase (RT) is an important drug target. RT has two activities, DNA synthesis, and cleavage of a DNA–RNA hybrid. These activities require that RT bind to both DNA and RNA. Single-molecule technology is used to show that the enzyme binds to RNA and DNA in different orientations, and provide insight into how it can flip between these orientations and activities without dissociating from the substrate.

    • Elio A. Abbondanzieri
    • Gregory Bokinsky
    • Xiaowei Zhuang
    Research
    Nature
    Volume: 453, P: 184-189

  • Efficient production of a simple carbapenem antibiotic in Escherichia coli is achieved by a combination of feedback-resistant enzymes for increased precursor biosynthesis and inhibition of fatty acid synthesis for tolerance toward the toxic product.

    • Helena Shomar
    • Sophie Gontier
    • Gregory Bokinsky
    Research
    Nature Chemical Biology
    Volume: 14, P: 794-800

  • The increasing cost of energy and concerns about the environment have emphasized the need to find new sources of fuel, with the microbial production of high-energy fuels a promising approach. Here, Escherichia coli is engineered to produce more complex biofuels — fatty esters (biodiesel), fatty alcohols and waxes — directly from simple sugars. Some cells are further engineered to express hemicellulases, a step towards producing these compounds directly from hemicellulose.

    • Eric J. Steen
    • Yisheng Kang
    • Jay D. Keasling
    Research
    Nature
    Volume: 463, P: 559-562