In this work, a substrate with dual anisotropy were prepared by combination of 3D printing and magnetic field-induced magnetic nanoparticle assembly. ADSCs cultured on this substrates have significantly higher osteogenic markers expression and more calcium nodules formation was also found, suggesting a stronger tendency of toward osteogenic differentiation of ADSCs. RNA-seq data revealed alteration of that alterations in kinase signaling pathway transduction, cell adhesion and cytoskeletalon reconstruction may account for the elevated osteogenic induction capacity.
