Synaptic vesicles are held in a fusion-competent state prior to their rapid release, which is thought to depend upon formation of trans-SNARE complexes. Takahashi et al. directly image this primed state using FLIM/FRET, and demonstrate differences in basal SNARE organization between neurons and β cells.
- Noriko Takahashi
- Wakako Sawada
- Haruo Kasai
